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The stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata? Herein, we report a simple and convenient method for the assessment of stomatal closing/opening in Arabidopsis thaliana based on fluorescent live imaging by Hoechst-tagged acetylfluorescein (HoeAc2Fl, Fig. 1a). Our method enables objective assessment of stomatal dynamics by simple monitoring of the fluorescence intensity of HoeAc2Fl in the nucleus of the stomata..Hoechst Sex Dating
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