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RNA was extracted using a tissue total RNA mini kit (FAVORGEN). Real-time quantitative PCR was performed using a KAPA SYBR Fast qPCR kit (KAPA BIOSYSTEMS) or a Taqman probe (ABI). Expression levels were normalized to that of the 18 S rRNA gene. Primer sequences are shown in Supplementary Data 1 in this article? The specific gene expression patterns in the liver of STAM mice were examined by Agilent Expression Array analysis on request of the researchers by Takara Bio Inc.Soeda Whore
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