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Compartment-specific pH monitoring in Bacillus subtilis using fluorescent sensor proteins: a tool to analyze the antibacterial effect of weak organic acids
While the PsspE-IpHluorin strain may also help understanding spore germination characteristics in the presence of potential outgrowth inhibitors such as the WOAs sorbic acid and acetic acid, clearly, because not all cells are in exactly the same state, these data represent the average value of the pHi in the population studies. To analyze the heterogeneity single-spore pHi measurements are needed. Currently we are extending our single cell live imaging tool “SporeTracker” (Pandey et al., 2013) to that end.Beilen Prostitute
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