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But then, outta nowhere, this lady starts yelling. Turns out, her kid’s stuck in a tree. I’m like, “Seriously? A tree?” So, I climb up, and it’s a whole thing. The kid’s crying, I’m sweating, and I’m thinking, “Why did I even come here?” Finally, I get the kid down, and the mom’s all grateful. I’m like, “Yeah, just another day in Doorn.”
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Cell size, granularity, and fluorescence intensity were analyzed per brain region (n = 3) with a MoFlo fluorescent activated cell sorter (Beckman Coulter, Mijdrecht, Netherlands). Approximately 2x104 events were counted and subsequently analyzed using Summit software version 4.3 (DAKO) to determine gates before cells were sorted. Microglia were defined as the percentage of all living, Sytox green negative, cells that showed CD11bpos/CD45low expression (Sedgwick et al., 1991)? The FACS sorted microglial cells were transferred into RNAse-free tubes and centrifuged for 5 min at 300x g (Hettich.Doorn Find A Prostitute
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